Fixation For Electron Microscopy

Download Read Online Fixation For Electron Microscopy book
Author: M.A. (Eric) Hayat
Publisher: Elsevier
ISBN: 0323147291
Size: 22.11 MB
Format: PDF, ePub, Mobi
View: 6407

Fixation For Electron Microscopy by M.A. (Eric) Hayat


Original Title: Fixation For Electron Microscopy

Fixation for Electron Microscopy presents how to better understand the effects of fixatives on the molecular structure of the cell. This book attempts to consider each aspect of fixation, including chemical interactions between fixatives and individual cellular substances. The chemistry of fixative interactions that are discussed in the book is based primarily on the reactions of a fixative with isolated proteins, lipids, nucleic acids, and carbohydrates. The book shows that the correct interpretation of information retrieved from electron micrographs depends on the knowledge of the basic principles underlying the fixation procedure. Also, the book presents the fixation of both eukaryotic and prokaryotic specimens. The special fixation conditions for plant specimens are discussed in detail and have been allotted a whole chapter. Also emphasized in this book is the connection between morphology and biochemical aspects of preparatory treatments and the chemical basis of the formation of artifacts. This topic is useful in understanding the modifications of cell structures introduced during their processing. A guide for recognizing and minimizing major artifacts and fixation faults that are usually encountered is also presented in the book. This valuable resource will prove useful to both students and professionals in the field of biology and clinical medicine. Specimen preservation researchers can also benefit from this book.

Practical Electron Microscopy

Download Read Online Practical Electron Microscopy book
Author: Elaine Evelyn Hunter
Publisher: Cambridge University Press
ISBN: 9780521385398
Size: 52.98 MB
Format: PDF, Kindle
View: 3692

Practical Electron Microscopy by Elaine Evelyn Hunter


Original Title: Practical Electron Microscopy

This is an extensively illustrated laboratory manual of transmission electron microscopy techniques for the laboratory technician, graduate student, or researcher. Chapters begin with a general discussion, move on to the chemicals and equipment required for the method being described and conclude with a step-by-step presentation of the method and instructions for the preparation of solutions. Notes at the end of each chapter warn of possible pitfalls and outline "tricks of the trade". The methods and techniques outlined have been tested for over ten years in clinical and research laboratory situations, and are entirely reliable. Practical Electron Microscopy covers fixation, dehydration and embedding, semithin and thin sectioning, the electron microscope, and photography. For this new edition, the chapters on photography and the electron microscope have been completely rewritten and two new chapters have been added, one on immunoelectron microscopy using colloidal gold, and one dealing with such special techniques as retrieving specimens from paraffin and handling nasal brushings and blood samples.

Principles And Techniques Of Electron Microscopy

Download Read Online Principles And Techniques Of Electron Microscopy book
Author: M. A. Hayat
Publisher:
ISBN: 9780521632874
Size: 74.25 MB
Format: PDF
View: 6512

Principles And Techniques Of Electron Microscopy by M. A. Hayat


Original Title: Principles And Techniques Of Electron Microscopy

Principles and Techniques of Electron Microscopy has established itself as a standard book for biological electron microscopists wishing to learn how to prepare their specimens for electron microscopic investigation. This fully revised and expanded 4th edition includes three new chapters covering such topics as immunocytochemistry and applications of microwave irradiation to microscopy. This revised and expanded 4th edition of Principles and Techniques of Electron Microscopy provides practical instructions on how to process biological specimens, as well as a detailed discussion on the principles underlying the various processes. The book presents methods in a self-explanatory form. Further, alternative procedures and points of disagreement help the reader interpret data accurately. What sets this book apart from its competition is that it not only describes techniques but also explains the principles behind the techniques; that is, chemical reactions underlying the use of various reagents for preserving and staining cellular components.

Biological Specimen Preparation For Transmission Electron Microscopy

Download Read Online Biological Specimen Preparation For Transmission Electron Microscopy book
Author: Audrey M. Glauert
Publisher: Princeton University Press
ISBN: 1400865026
Size: 61.23 MB
Format: PDF, ePub, Docs
View: 6237

Biological Specimen Preparation For Transmission Electron Microscopy by Audrey M. Glauert


Original Title: Biological Specimen Preparation For Transmission Electron Microscopy

This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Biological Electron Microscopy

Download Read Online Biological Electron Microscopy book
Author: Michael J. Dykstra
Publisher: Springer Science & Business Media
ISBN: 146840010X
Size: 55.51 MB
Format: PDF, Docs
View: 6327

Biological Electron Microscopy by Michael J. Dykstra


Original Title: Biological Electron Microscopy

In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.

A Manual Of Applied Techniques For Biological Electron Microscopy

Download Read Online A Manual Of Applied Techniques For Biological Electron Microscopy book
Author: Michael J. Dykstra
Publisher: Springer Science & Business Media
ISBN: 9780306444494
Size: 77.21 MB
Format: PDF, ePub, Mobi
View: 3443

A Manual Of Applied Techniques For Biological Electron Microscopy by Michael J. Dykstra


Original Title: A Manual Of Applied Techniques For Biological Electron Microscopy

A cookbook of techniques used routinely in a service-oriented electron microscopy laboratory on a broad variety of tissue and cell samples. For each technique, describes the problem to be addressed, the formulation of stock solutions, specific procedural steps, and the results expected. To be used i

Biomedical Electron Microscopy

Download Read Online Biomedical Electron Microscopy book
Author: Arvid B. Maunsbach
Publisher: Elsevier
ISBN: 9780080528090
Size: 78.94 MB
Format: PDF, Kindle
View: 916

Biomedical Electron Microscopy by Arvid B. Maunsbach


Original Title: Biomedical Electron Microscopy

This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. Authored by the key leaders in the biological electron microscopy field Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines Introduces students on how to read and interpret electron micrographs

Introduction To Electron Microscopy

Download Read Online Introduction To Electron Microscopy book
Author: Saul Wischnitzer
Publisher: Elsevier
ISBN: 1483148696
Size: 60.55 MB
Format: PDF, Mobi
View: 4162

Introduction To Electron Microscopy by Saul Wischnitzer


Original Title: Introduction To Electron Microscopy

Introduction to Electron Microscopy, Second Edition provides an introduction to the foundations of electron microscopy; an outline of some practical aspects of instrument operation; and discussion of the rationale of the methodology of biological specimen preparation. The book seeks to provide a comprehensive understanding of the theoretical and operational aspects of the electron microscope. This edition consists of two parts. Part One deals with the history, basic theory, and operation of the electron microscope. Part Two discusses steps used in material preparation for electron microscope investigation such as fixation, embedding, and staining techniques. Biomedical researchers, molecular biologists, toxicologists, forensic investigators, and medical students will find this book a very useful reference.

Recent Books :